Biolegend, 648818, Ultra-LEAF™ Purified anti-mouse IL-33 Antibody, 100μg

IL-33 belongs to the IL-1 family with close structural similarities with IL-18 and IL-1β. IL-33, IL-1β, and IL-18 are synthesized as biologically inactive precursor. These in-active precursors are released in their active mature form after undergoing cleavage by caspase-1 enzyme. Like IL-1β, IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 to activate NF-κB and MAPK pathways. In addition, IL-33 can also act as a nuclear factor, known as NF-HEV. Exhibiting transcriptional regulatory properties, NF-HEV been demonstrated to being abundantly expressed in the nucleus of high endothelial venules of lymphoid organs that are associated with chromatin.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Mouse IL-33
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.5 µg/mL of mouse IL-1RL1 (ST2)-Fc (Cat. No. 764902) to immobilized 3 µg/mL recombinant mouse IL-33 (Cat. No. 580506). ND50 range is 0.2 - 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3720525 (BioLegend Cat. No. 648818) AB_3720525 (BioLegend Cat. No. 648819)
Structure: Monomer
Distribution: High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues.
Function: IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-α and IL-1β are activators of IL-33 transcription in fibroblasts and keratinocytes.
Interaction: Th2 lymphocytes, CD8 cells, mast cells, macrophages, dendritic cells (DCs), eosinophils, basophils, endothelial, and epithelial cells
Ligand/Receptor: IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein)
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: Schmitz J, et al. 2005 Immunity. 23:479. Barksby HE, et al. 2007. C Exp Immunol. 149:217. Arend WP, et al. 2008. ImmRev. 223:20. Suzukawa M, et al. 2008. J. Immunol. 181:5981. Moussion C, et al. 2008. PloS One 2008. 10:e3331. Carriere V, et al 2007. Proc Natl Acad Sci U S A. 104:282.
Gene ID: 77125
UniProt: View information about IL-33 on UniProt.org
Clone: A23126A
Regulatory Status: RUO
Other Names: Il-1f11, Nuclear factor from high endothelial venules (NF-HEV)
Isotype: Rat IgG2b, λ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.