Biolegend, 648820, Purified anti-MRP-14 (S100A9) Antibody, 25μg

The S100A8/A9 complex has been shown to play a role in calcium-dependent activation and exhibits antibacterial, antifungal, immunomodulatory, and anti-proliferative effects. It also functions as a chemoattractant for neutrophils and serves as an endogenous activator of Toll-like receptor 4 (TLR4). S100A9 has been identified as a marker of CD14⁺ HLA-DR⁻/low myeloid-derived suppressor cells (MDSCs).
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein of human MRP-14 (S100A9)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol. For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Fixation/permeabilization with either True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.
RRID: AB_3720530 (BioLegend Cat. No. 648820) AB_3720530 (BioLegend Cat. No. 648821)
Structure: MRP-14 (S100A9) s is a 114 amino acid protein with a predicted molecular weight of 13.2 kD.
Distribution: Secreted and localized to the cytoplasm and cell membrane in cells such as MDSCs, neutrophils, monocyte, and ileal tissue eosinophil.
Function: Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Cell Type: Eosinophils, Monocytes, Neutrophils
Biology Area: Cancer Biomarkers, Cell Biology, Immunology, Innate Immunity
Antigen References: Loser K, et al. 2010. Nat. Med. 16:713. Cheng P, et al. 2008. J. Exp. Med. 205:2235. Leukert N, et al. 2006. J. Mol. Biol. 359:961. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402. Zhao F, et al. 2012. Immunology. 136:176.
Gene ID: 6280
UniProt: View information about MRP-14 on UniProt.org
Clone: A22135C
Regulatory Status: RUO
Other Names: S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype: Mouse IgG2b, κ