Biolegend, 648835, Purified anti-CTBP2 Antibody, 100μg

C-terminal-binding protein 2, also known as CTBP2, is a transcriptional co‑repressor, originally discovered to bind to the carboxy-terminus of the human adenovirus E1A protein. CTBP family proteins are encoded by 2 paralogous genes, CTBP1 and CTBP2. CTBPs promote cancer cell migration, apoptosis antagonists, and tumor suppressor genes repressors. CTBP2 has been previously identified as being capable of directing cell migration by repressing PTEN expression and stimulating PI3K activity, which leads to Rac-dependent migration in some cancers. However, another study revealed that inhibiting CTBP2 enhanced stemness and the occurrence of primary ovarian carcinoma, while higher levels of CTBP2 expression correlate with improved overall survival (OS). These observations suggest that CTBP2 has two biological roles in influencing by different partner genes and downstream regulations. Furthermore, the investigations discovered that CTBP2 has a significant correlation between cell metabolism and the etiology of obesity in people.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CTBP2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: The clone cross-reacts with mouse in western blot (WB).
RRID: AB_3720545 (BioLegend Cat. No. 648834) AB_3720545 (BioLegend Cat. No. 648835)
Structure: CTBP2 is a 445 amino acid protein with a predicted molecular weight of 49 kD.
Distribution: Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. Found in nucleus and synapse.
Function: Transcription regulators
Interaction: HIPK2, ZNF217, PRDM16, PNN, NRIP1, WIZ and MCRIP1.
Cell Type: Astrocytes, Microglia, Neurons
Biology Area: Cardiovascular Biology, Cell Biology, Neuroscience
Antigen References: Chinnadurai G. 2009. Cancer Res. 69:731. Boyd JM, et al. 1993. EMBO J. 12:469. Katsanis, N. and Fisher, E.M. 1998. Genomics. 47:294. Paliwal S, et al. 2007. Cancer Res. 67:9322. Tracy-X C, et al. 2013. Immunity. 39:611. Sekiya M, et al. 2024. J Atheroscler Thromb. 31:109.
Gene ID: 1488
UniProt: View information about CTBP2 on UniProt.org
Clone: W23149C
Regulatory Status: RUO
Other Names: C-Terminal Binding Protein 2, CtBP2
Isotype: Rat IgG2a, κ