Biolegend, 648848, Purified anti-STAT4 Antibody, 25μg

Signal Transducer and Activator of Transcription 4 (STAT4) is a critical transcription factor involved in mediating immune responses. STAT4 is primarily activated via phosphorylation which leads to its dimerization, nuclear translocation, and subsequent activation of target genes involved in immune regulation. STAT4 plays a pivotal role in the differentiation of T helper 1 (Th1) cells, follicular helper T cells (Tfh), and regulatory T cells (Tregs), and is essential for the function of natural killer (NK) cells and CD8+ T cells. STAT4 expression is tightly regulated and varies across cell types and activation states, with higher expression observed in activated immune cells such as mature dendritic cells and monocytes. Genetic polymorphisms in the STAT4 gene, such as H623Y and A635V, have been linked to altered STAT4 activity and nuclear localization, influencing susceptibility to various diseases, such as rheumatoid arthritis, systemic lupus erythematosus, type 1 diabetes, and inflammatory bowel disease.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human STAT4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either of the following: -Citrate Buffer (Cat. No. 420902) -Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704)
RRID: AB_3720575 (BioLegend Cat. No. 648848) AB_3720575 (BioLegend Cat. No. 648849)
Structure: The STAT4 protein is composed of 748 amino acids and has a molecular weight of approximately 86 kDa.
Distribution: STAT4 is primarily located in the cytoplasm of cells in its inactive form. Upon activation, dimerizes and translocates to the nucleus.
Cell Type: Dendritic cells, Lymphocytes, Neurons, NK cells, NKT cells, T cells
Biology Area: Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Neuroscience, Signal Transduction, Transcription Factors
Molecular Family: Innate Immune Signaling, Phospho-Proteins
Antigen References: Xia Y, et al. 2024. Front Immunol. 15:1479418. Tolomeo M, et al. 2024. Int Rev Immunol. 43(6):394. Yang c, et al. 2020. Int J Biol Sci. 16(9):1575. Wurster AL, et al. 2000. Oncogene. 19(21):2577. Buzzelli AA, et al. 2023. J Immunol. 210(11):1667.
Gene ID: 6775
UniProt: View information about STAT4 on UniProt.org
Clone: W22042B
Regulatory Status: RUO
Other Names: Signal Transducer and Activator of Transcription 4, SLEB11, DPMC
Isotype: Rat IgG2a, κ