Biolegend, 648862, Ultra-LEAF™ Purified anti-human Angiopoietin-2 Antibody, 100μg

Angiopoietin-2, also known as ANGPT2, is a member of angiopoietins, which plays essential roles in embryonic and postnatal angiogenesis. The structure of the mature form of ANGPT2 contains an N-terminal coiled-coil domain, which mediates multimerization of the protein, along with a C-terminal fibrinogen-like domain, which mediates the receptor binding. Both ANGPT1 and ANGPT2 are ligands for the receptor tyrosine kinase, Tie-2. ANGPT2 appears to exist predominantly as a homodimer, but it is also capable of forming multimers while ANGPT1 predominantly exists as multimers. It has been shown that ANGPT2 is able to promote vascular leakage and neutrophil migration. ANGPT2 can also sensitize TNF-α-induced monocyte adhesion, thus it is considered a proinflammatory factor. Although ANGPT2 is not required for arterial vascular development, its expression in the tissue suggests that it may play a role in the maintenance of these vessels. This conclusion is also supported by the observation that high concentrations of ANGPT2 activate the same survival signaling pathway as ANGPT1 to prevent endothelial cell apoptosis via Tie-2/Akt. Additionally, ANGPT2 was demonstrated to function as an agonist in the absence of ANGPT1, but in its presence it acts as an antagonist. Human ANGPT2 shares 86% amino acid sequence homology with its murine counterpart.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human Angiopoietin-2
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.25 µg/mL human Angiopoietin-2 to immobilized 1.5 µg/mL recombinant human Tie-2-Fc (Cat. No. 784302). ND50 range: 0.015 – 0.15 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3720596 (BioLegend Cat. No. 648862) AB_3720596 (BioLegend Cat. No. 648863)
Structure: Oligomer
Distribution: Placenta, uterus, ovaries, and vascular endothelial cells
Function: ANGPT2 plays a role in angiogenesis and monocyte adhesion. Hyperoxia induces ANGPT2 expression in the lung epithelial cells.
Interaction: Monocytes and endothelial cells
Ligand/Receptor: Tie-2
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Augustin HG, et al. 2009. Nat. Rev. Mol. Biol. 10:165. Ward NL, Dumont DJ. 2002. Semin Cell Dev Biol. 1:19. Roviezzo F, et al. 2005. J. Pharmacol. Exp. Ther. 314:738. Sturn DH, et al. 2005. Microcirculation. 12:393. Murdoch C, et al. 2007. J. Immunol. 178:7405. Yuan HT, et al. 2009. Mol. Cell Biol. 29:2011. Procopio WN,et al. 1999. J. Biol. Chem. 274:30196. Bhandari V, et al. 2006. Nature Med. 12:1286.
Gene ID: 285
UniProt: View information about Angiopoietin-2 on UniProt.org
Clone: W19419A
Regulatory Status: RUO
Other Names: Angiopoietin 2, ANG2, Ang-2, ANGPT2
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.