Biolegend, 648879, Purified Anti-Musashi-2 Antibody, 100μg

Musashi-2 (MSI2) is a highly conserved RNA-binding protein that plays a critical role in post-transcriptional regulation of gene expression, particularly in stem cell maintenance, differentiation, and proliferation. Musashi-2 is essential for normal hematopoiesis and neural development, where it regulates the translation of mRNAs involved in cell cycle and developmental pathways. Dysregulation has been implicated in a variety of cancers, such as acute myeloid leukemia (AML) and MLL-rearranged acute lymphoblastic leukemia (ALL), where MSI2 supports leukemic stem cell survival and contributes to therapy resistance. Musashi-2 also promotes tumor progression in solid malignancies like pancreatic cancer, by modulating pathways such as PI3K/AKT/mTOR and epithelial-mesenchymal transition (EMT). Elevated Musashi-2 expression is frequently associated with poor prognosis, increased metastasis, and resistance to conventional therapies. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 WT Cell Line (Horizon Cat No. Cat. No. C631) and HAP1 MSI2 Knockout cell line (Horizon Cat. No. HZGHC006122c011)
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Musashi-2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). Similar results were also obtained using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803). For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).
RRID: AB_3720622 (BioLegend Cat. No. 648878) AB_3720622 (BioLegend Cat. No. 648879)
Structure: Musashi-2 protein consists of 328 amino acids and has a molecular weight of approximately 35.2 kDa.
Distribution: Cytoplasm
Interaction: RHOBTB2, HNRNPH1 and HNRNPH2, MAPT (Tau protein), MEOX2, TIMM10B
Cell Type: Hematopoietic stem and progenitors, Leukemia, Monocytes, Neural Stem Cells, T cells
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, DNA Repair/Replication
Antigen References: Huang L, et al. 2024. Cancer Cell Int. 24(1):273. Jiang L, et al. 2022. Front Oncol. 12:969632. Zang H, et al. 2025. Leukemia. 39(1):265. Taggart J, et al. 2016. Nat Commun. 7:10739.
Gene ID: 124540
UniProt: View information about Musashi-2 on UniProt.org
Clone: W23272C
Regulatory Status: RUO
Other Names: MSI2, MSI2H, Musashi-2, Musashi RNA Binding Protein 2, RNA-Binding Protein Musashi Homolog 2
Isotype: Rat IgG2b, κ