Biolegend, 649152, Purified anti-CDC2/CDK1 Phospho (Thr161) Antibody, 100μg

CDC2, also known as CDK1 or Cyclin-Dependent Kinase 1, is a crucial regulator of G2 phase progression to mitosis in the cell cycle. The phosphorylation of CDC2 at Threonine 161 (Thr161) is essential for its activation. This phosphorylation is carried out by the CDK-activating kinase (CAK) and is necessary for CDC2 to form an active complex with cyclin B1, promoting mitosis. The regulation of CDC2 activity involves a delicate balance of phosphorylation and dephosphorylation events. In maintaining this balance, kinases like WEE1 and MYT1 inhibit CDC2 by phosphorylating other residues. Meanwhile, phosphatases like CDC25 activate CDC2 by removing these inhibitory phosphates. Dysregulation of CDC2, including aberrant phosphorylation at Thr161, is associated with various cancers, such as breast, liver, and colon cancers.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human CDK1 phosphorylated at threonine 161
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has cross reactivity with CDK2 phospho (Thr160) (verified), CDK3 phospho (Thr172) (verified), and CDK7 phospho (Thr170) (expected, not verified) due to the high homology/identity with CDK1 phospho (Thr161) site.
Additional Product Notes: For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3717197 (BioLegend Cat. No. 649151) AB_3717197 (BioLegend Cat. No. 649152)
Structure: CDC2 is 297 amino acids length protein with a molecular weight of approximately 34 kDa.
Distribution: Nucleoplasm and cytoplasm
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Morgan D. 1995. Nature. 374:131. Massacci G, et al. 2023. British Journal of Cancer. 129:1707. Enserink JM, et al. 2010. Cell Division. 5:1. Cho Y, et al. 2025. Cell Death Dis. 16:202.
Gene ID: 983
UniProt: View information about CDC2/CDK1 on UniProt.org
Clone: A23004F
Regulatory Status: RUO
Other Names: CDC2: Cell Division Cycle 2 homolog12, CDC28A, P34CDC2, Cell Division Protein Kinase 1:
Isotype: Mouse IgG1, κ