Biolegend, 649551, Purified anti-CD4 Antibody, 25μg

CD4 is a single-chain type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR (T-Cell Receptor)/CD3 complex and binds to the β2 domain of the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the extracellular domain of human CD4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). Staining intensity may be stronger using Citrate Buffer. This product is not recommended for use in immunocytochemistry (ICC) or intracellular flow cytometry (ICFC). This product does not react with mouse CD4 in Western Blot (WB) or immunohistochemistry on frozen tissues (IHC-F).
RRID: AB_3675180 (BioLegend Cat. No. 649551) AB_3675180 (BioLegend Cat. No. 649552)
Structure: CD4 is a 458 amino acid protein with a predicted molecular weight of 51.1 kD.
Distribution: Plasma membrane
Function: Co-receptor
Interaction: Part of the TCR/CD3 complex
Ligand/Receptor: Β2 domain from MHC class II, IL-16
Cell Type: Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Zhu J, et al. 2010. Annu Rev Immunol. 28:445. Vignali DA. 2010. J. Immunol. 184:5933. Zhou L, et al. 2009. Immunity. 30:646. Singer A, et al. 2008. Nat Rev Immunol. 8:788. Zhu J and Paul WE. 2008. Blood. 112:1557.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: W23082E
Regulatory Status: RUO
Other Names: T4, Leu3, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Isotype: Rat IgG2b, κ