Biolegend, 650202, Purified anti-SIRT2 Antibody, 100μg

Mammalian sirtuin family proteins are homologs of the Saccharomyces cerevisiae silencing protein Sir2. Sirtuins are NAD-dependent deacylases and ADP-ribosyl transferases involved in the regulation of various cellular processes, such as metabolism, autophagy, apoptosis, gene silencing, and lifespan. Unlike the other sirtuin members, Sirtuin 2 (SIRT2) is predominantly distributed in the cytoplasm and functions as a microtubule deacetylase. SIRT2 also deacetylates lysines on histone and several key transcriptional factors, including P53 and NF-κB. SIRT2 shuttles continuously between cytoplasmic and nuclear during interphase, associates with chromatin in early prophase, and localizes to centrosomes and the mitotic spindle during metaphase. SIRT2 has been implicated in the pathology of cancer and neurodegenerative diseases. SIRT2 deacetylates histone proteins and prevents chromosomal instability during mitosis. Inhibition of SIRT2 has been shown to negatively regulate polyglutamine inclusion and rescue neuronal cells from α-synuclein-induced toxicity.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human SIRT2 recombinant protein (140-389 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: As per the Human Protein Atlas RNA level dataset, NTERA2 cell is expected to have a low level of SIRT2 expression.Human SIRT2 has 5 isoforms with Mw ranging from 30kDa to 43kDa.This clone cannot be used for IF application (in house tested).
RRID: AB_2721752 (BioLegend Cat. No. 650201) AB_2721753 (BioLegend Cat. No. 650202)
Structure: SIRT2 have five reported isoforms, with the predicted molecular weight of 43.2, 41.3, 39.5, 35.7, and 30.4 kD. Contains a sirtuin-type deacetylase domain.
Distribution: Cytoplasm, Nucleus.
Function: Sirtuin 2 (SIRT2) is an NAD-dependent deacetylase known to participates in the modulation of multiple biological processes, including cell cycle progression, genomic stability, microtubule dynamics, NAD metabolism, and autophagy.
Interaction: Interacts with CDC20, FOXO3, FZR1, HDAC6, FOXO1, RELA, HOXA10, YWHAB, YWHAG, ERK1/2, KMT5A, G6PD, AURKA, CDK5R1, CDK5, SARS, and HIF1A.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Jęśko H, et al. 2017. Neurochem Res. 42:876. 2. Cosentino C, et al. 2014. EMBO J. 33:1417. 3. Nagai T, et al. 2013. J Cell Sci. 126:4369. 4. Donmez G, et al. 2013. EMBO Mol Med. 5:344. 5. Cha YI, et al. 2013. BMB Rep. 46:429. 6. Inoue T, et al. 2007. Cell Cycle. 6:1011.
Gene ID: 22933
UniProt: View information about SIRT2 on UniProt.org
Clone: W16205A
Regulatory Status: RUO
Other Names: Sirtuin 2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2 (SIR2L2), SIR2L
Isotype: Rat IgG2a, κ