Biolegend, 650804, Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10) Antibody, 100tests

Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Modified synthetic peptide conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.
Product Citations: Caiazza F, et al. 2019. Am J Pathol. 189:1916. PubMed Jackson TR, et al. 2022. iScience. 25:104787. PubMed Chou DB, et al. 2022. Nat Commun. 13:6021. PubMed Frendo-Cumbo S, et al. 2022. iScience. 25:105188. PubMed Amar-Schwartz A, et al. 2022. Elife. 11:. PubMed
RRID: AB_10917386 (BioLegend Cat. No. 650803) AB_10918435 (BioLegend Cat. No. 650804)
Structure: H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution: Nucleus
Function: H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Interaction: Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: 1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202. 4. Hans F, et al. 2001. Oncogene 20:3021.
Regulation: H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID: 8290
UniProt: View information about Histone on UniProt.org
Clone: 11D8
Regulatory Status: RUO
Other Names: Histone-H3, H3
Isotype: Mouse IgG2b, κ