Biolegend, 651951, Purified anti-CD14 Antibody, 25μg

CD14 is a glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. It plays an important role in the innate immune system, and is the first pattern recognition receptor identified. CD14 primarily binds to bacterial lipopolysaccharide (LPS), a component of the outer membrane of Gram-negative bacteria. By binding to LPS, CD14 initiates a signaling cascade that leads to the clearance of pathogens.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant peptide containing the full-length extracellular domain of human CD14
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody (clone W23074F) in immunohistochemistry with formalin-fixed, paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with either Sodium Citrate (Cat. No. 928502), or Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). This antibody (clone W23074F) is not suitable for western blot, flow cytometry or immunocytochemistry.
RRID: AB_3662331 (BioLegend Cat. No. 651951) AB_3662331 (BioLegend Cat. No. 651952)
Structure: CD14 is a 375 amino acid protein with a predicted molecular weight of 40.1 kD. Glycosylated/modified CD14 has a predicted molecular weight of ~53 kD.
Distribution: Cell surface/plasma membrane
Function: Receptor
Interaction: TLR4, MD-2
Ligand/Receptor: Bacterial lipopolysaccharide (LPS)
Cell Type: Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: Stocks S, et al. 1990. Biochem. J. 268:275. Wright S, et al. 1990. Science. 249:1434. Sharygin D. et al. 2023. Immunology. 169:260-270.
Gene ID: 929
UniProt: View information about CD14 on UniProt.org
Clone: W23074F
Regulatory Status: RUO
Other Names: Monocyte differentiation antigen CD14, P08571, Myeloid cell-specific leucine-rich glycoprotein, Lipopolysaccaride (LPS) receptor
Isotype: Rat IgG2b, κ