Biolegend, 652513, Alexa Fluor® 647 anti-His Tag Antibody, 25μg

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).
25μg
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated His-tag peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml (Dilution 1:1000-1:2500) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations: Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed Yusuf B, et al. 2021. Sci Rep. 11:3614. PubMed Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
RRID: AB_2716153 (BioLegend Cat. No. 652513)
Function: Recombinant poly-histidine tag detection
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about His Tag on UniProt.org
Clone: J099B12
Regulatory Status: RUO
Other Names: Histidine Tag
Isotype: Mouse IgG1, κ