Biolegend, 653102, Purified anti-LEF1 Antibody, 100μg

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human LEF1 recombinant protein (1-298 aa)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL (1:5000 - 1:500). For immunoprecipitation, the suggested use of this reagent is 5 - 20 μg per mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: LEF-1 is known to have 7 isoforms. IHC-P (Immunohistochemistry FFPE) is not suggested for this product. For ICFC (intracellular flowcytometry), True-Phos™ Perm Buffer (Cat. No. 425401) is suggested for permeabilization.
Product Citations: Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed
RRID: AB_2561614 (BioLegend Cat. No. 653101) AB_2561615 (BioLegend Cat. No. 653102)
Structure: 44 kD protein containing a DNA-binding domain (HMG box), an CTNNB1-binding region, and a Prolin-rich domain
Distribution: Nucleus
Function: LEF1 is a transcription factor mediating canonical Wnt signaling pathway. It has been shown to function in hair bud formation, cancer development, tumorigenesis, angiogenesis, and T-cell maturation.
Interaction: Interacts with ALX4, AML-1, beta-catenin (CTNNB1), EP300, MITF, PIAS4, SMAD2, and SMAD3
Biology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers, TCRs
Antigen References: Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184. Waterman ML, et al. 1991. Genes Dev. 5:656. Hovanes K, et al. 2001. Nat Genet. 28:53. Bruhn L, et al. 1997. Genes Dev. 11:640. Nguyen A, et al. 2005. Int. J. Oncol. 27:949. Petropoulos K, et al. 2008. J. Exp. Med. 205:515.
Gene ID: 51176
UniProt: View information about LEF1 on UniProt.org
Clone: 15H5A18
Regulatory Status: RUO
Other Names: Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha (TCF1-alpha)
Isotype: Mouse IgG1, κ