Biolegend, 653304, PE anti-mouse Ikaros Antibody, 100μg

Ikaros family members, including Ikaros and Helios, play a role in the development of lymphocytes and B and T cells. They are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and IL-2 mediated signaling events.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal half of mouse Ikaros fused to GST.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported references (for the relevant formats) include: immunofluorescence1 and immunoprecipitation2.Does not cross-react with human.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).View more applications data for a different format of this clone in our Scientific Poster Library.This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.
Application References(PubMed link indicates BioLegend citation): Cobb SB, et al. 2000. Genes & Dev. 14:2146. (IF) Hahm K. et al. 1998. Genes Dev. 12:782. (IP) Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed
Product Citations: Guenther C, et al. 2024. Front Immunol. 1434030:15. PubMed Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed
RRID: AB_2561727 (BioLegend Cat. No. 653303) AB_2561728 (BioLegend Cat. No. 653304)
Structure: Belongs to the Ikaros C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers. 519 amino acids with predicted MW of 58 kD.
Distribution: Nucleus. Abundantly expressed in thymus, spleen and peripheral blood leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
Function: Transcription regulator of hematopoietic cell differentiation. Plays a role in the development of lymphocytes, B and T cells.
Interaction: Heterodimer with other IKAROS family members.
Cell Targets: Component of the chromatin-remodeling NuRD repressor complex which includes at least HDAC1, HDAC2, RBBP4, RBBP7, IKZF1, MTA2, MBD2, MBD3, MTA1L1, CHD3, and CHD4. Interacts directly with the CHD4 component of the NuRD complex.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. John LB and Ward AC, 2011. Mol. Immunol. 48:1272. 2. Merkenschlager M, 2010. FEBS Lett. 584:4910. 3. Thomas RM, et al. 2010. J. Biol. Chem. 285:2545. 4. Papathanasiou P, et al. 2009. Stem Cell. 27:3082. 5. Molnar A, et al. 1996. J. Immunol. 156:585.
Gene ID: 22778
UniProt: View information about Ikaros on UniProt.org
Clone: 2A9/Ikaros
Regulatory Status: RUO
Other Names: IKAROS family zinc finger 1, DNA-binding protein Ikaros
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.