Biolegend, 653501, Purified anti-Nur77 Antibody, 25μg

Nur77, a transcription factor belonging to a superfamily of orphan nuclear receptors, has been shown to activate a subset of genes containing NGFI-B response element (NBRE). Nur77 expression is upregulated in response to stimuli of numerous growth factors, mitogens, and inflammatory substances, indicating that Nur77 has regulatory roles in cell growth and inflammation. Nur77 has also been shown to participate in programmed cell death through mitochondrial targeting and interaction with Bcl-2. In the endocrine system, Nur77 has been reported to participate in regulating production of steroidogenic enzymes. Nur77 and NOR-1 double knockout mice develop acute myeloid leukemia, suggesting that Nur77 plays a role in regulating differentiation of myeloid progenitor cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human Nur77 recombinant protein (1-266 aa)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1 - 0.5 µg/mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2561762 (BioLegend Cat. No. 653501) AB_2561763 (BioLegend Cat. No. 653502)
Structure: 64 kD protein containing a DNA-binding domain, two zinc finger domain, and a potential ligand-binding domain
Distribution: Nucleus
Function: Nur77 is a transcription factor that mediates various physiological stimuli, such as growth factors, hormones, inflammatory, and energy metabolic signals. Nur77 plays roles in many important biological processes including cell growth, differentiation, and apoptosis.
Interaction: Interacts with GADD45GIP1 and Bcl-2.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Nuclear Markers, Steroid Receptors/Nuclear Receptors
Antigen References: 1. Nakai A, et al. 1990. Mol. Endocrinol. 4:1438. 2. Han YH, et al. 2006. Oncogene 25:2974. 3. Li M, et al. 2010. Mol. Cell. Endocrinol. 319:39. 4. Pearen MA, et al. 2010. Mol. Endocrinol. 24:1891. 5. Moll UM, et al. 2006. Oncogene 25:4725. 6. Fassett MS, et al. 2012. Proc. Natl. Acad. Sci. USA 109:3891.
Gene ID: 3164
UniProt: View information about Nur77 on UniProt.org
Clone: 1E10A15
Regulatory Status: RUO
Other Names: Nuclear receptor subfamily 4, group A, member 1 (NR4A1), GFRP, HMR, Testicular receptor 3 (TR3)
Isotype: Mouse IgG2b, κ