Biolegend, 654101, Purified anti-TIF1β (KAP-1, TRIM28) Phospho (Ser473) Antibody, 25μg

Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. TIF1β is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Modified peptide: VKRSRpSGEGEVC.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Jamal K, et al. 2022. Cancer Res Commun. 2:1244. PubMed Boonen RACM, et al. 2022. Cancer Res. 82:615. PubMed Storchova R, et al. 2021. FEBS J. 288:6035. PubMed Zu R, et al. 2021. Cell Death Dis. 12:994. PubMed
RRID: AB_2561781 (BioLegend Cat. No. 654101) AB_2561782 (BioLegend Cat. No. 654102)
Structure: An N-terminal RING-B box coiled coil (RBCC) domain most likely attributes to intermolecular structure, while two characteristic chromatin-targeting domains, a plextrin homology (PH) finger motif and bromodomain, are found in the C-terminus.
Distribution: Nucleus
Function: TIF1β acts as a transcriptional repressor by modulating histone deacetlyation, histone H3 Ly-9 methylation, and recruitment of HP1 proteins.
Interaction: TIF1β was shown to form homo-oligomers in vitro, but does not form hetero-oligomers with TIF1α or TIF1γ.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: 1. Messerschmidt DM, et al. 2012. Science 335:1499. 2. Chikuma S, et al. 2012. Nat. Immunol. 13:596.
Gene ID: 21849
UniProt: View information about TIF1beta on UniProt.org
Clone: 11G10SC
Regulatory Status: RUO
Other Names: TRIM28 pS473, KAP-1 pS473
Isotype: Mouse IgG1, κ