Biolegend, 654702, Purified anti-SWAP70 Antibody, 100μg

SWAP70 was initially isolated as a component of an isotype switch recombination complex (SWAP) from activated B lymphocytes. It translocates from cytoplasm to the nucleus upon B cell activation. SWAP70 interacts with F-actin and RAC, binds to PI(3,4)P 2 , and regulates membrane ruffling and lamellipodia formation in response to growth factor stimulation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human SWAP70 recombinant protein (89-585 aa).
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: Affinity purified.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 10-30 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2561890 (BioLegend Cat. No. 654702)
Structure: Contains a PH domain, which is required for binding of phosphatidylinositol 3,4,5-trisphosphate, a coil-coil domain, and a domain weakly homologous to DH domains. Theoretical molecular weight is 69 kD.
Distribution: SWAP70 is localized to cytoplasm in resting B-cells, recruited to plasma membrane, and translocated to the nucleus upon B-cell activation.
Function: SWAP70 is a guanine nucleotide exchange factor (GEF) which binds phosphatidylinositol trisphosphate and transduces signals from tyrosine kinase receptors to RAC. SWAP70 is required for membrane ruffling and lamellipodia formation during cytoskeletal F-actin rearrangement.
Interaction: Interacts with cytoskeletal F-actin and Rac to regulate actin remodeling. Associates with NPM1, NCL, and PARP1 in switch-associated protein complex.
Cell Type: B cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction
Antigen References: 1. Masat L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:2180. 2. Hilpelä P, et al. 2003. Mol. Biol. Cell. 14:3242. 3. Ihara S, et al. 2006. J. Cell Sci. 119:500. 4. Shinohara M, et al. 2002. Nature 416:759. 5. Pearce G, et al. 2006. Nat. Immunol. 7:827.
Gene ID: 23075
UniProt: View information about SWAP70 on UniProt.org
Clone: 9D9A19
Regulatory Status: RUO
Other Names: SWAP switching B-cell complex 70 kD subunit, switch-associated protein 70
Isotype: Mouse IgG1, κ