Biolegend, 655202, Purified anti-TCF1 (TCF7) Antibody, 100μg

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial TCF1 recombinant protein (116-334 aa)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Affinity purified
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per mL. For immunocytochemmistry microscopy, a concentration range of 1-5 µg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed Sekine T, et al. 2020. Sci Immunol. 5: . PubMed Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
RRID: AB_2562103 (BioLegend Cat. No. 655202)
Structure: 384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
Distribution: Nucleus
Function: TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Interaction: TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers, TCRs
Antigen References: 1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207. 2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060. 3. Wang R, et al. 2011. J. Immunol. 187:5964. 4. Weber BN, et al. 2011. Nature 476:63. 5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777. 6. Staal FJ, et al. 1999. Int. Immunol. 11:317.
Gene ID: 6932
UniProt: View information about TCF1 on UniProt.org
Clone: 7F11A10
Regulatory Status: RUO
Other Names: T-cell-specific transcription factor 1, transcription factor 7, TCF7
Isotype: Mouse IgG1, κ