Biolegend, 655902, Purified anti-Wntless (GPR177) Antibody, 100μg

Wnt signaling plays important roles in embryonic development and cancer formation. Wntless (GPR177) is a putative G-protein coupled receptor that regulates Wnt's trafficking and secretion. It can regulate the expression, subcellular location, binding, and organelle-specific association of Wnt proteins. In mammals, Wntless is mainly expressed in submucosa, muscle, ganglia, and nerve cells of the large intestines. Overexpression of Wntless may activate the NF-κB signaling pathway in cancer cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Wntless protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone recognizes a ~65 kD protein of unidentified origin. Additional reported applications (for relevant formats) include: immunoprecipitation (IP)1 and immunocytochemistry (ICC)1
Application References(PubMed link indicates BioLegend citation): Coombs GS, et al. 2010. J. Cell Sci. 123:3357. (ICC, IP)
Product Citations: Wesslowski J, et al. 2020. J Biol Chem. 295:8759. PubMed Glaeser K, et al. 2018. EMBO J. 37:. PubMed
RRID: AB_2562228 (BioLegend Cat. No. 655902)
Structure: Three isoforms with 450 to 543 amino acids, predicted molecular weight of 45-65 kD; structure similar to the member of G-protein coupled receptor (GPCR) superfamily, with conserved YXXΦ endocytotic motif
Distribution: Cytoplasmic organelles
Function: Regulates Wnt protein sorting and secretion; it plays a key role in the regulation of expression, subcellular localization, binding and organelle-specific association of Wnt proteins
Interaction: Interacts with WNT1, WNT3, WNT5A, and Vps35
Ligand/Receptor: Wnt
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Signal Transduction
Antigen References: 1. Hardin J and King RS, et al. 2008. Curr. Opin. Genet. Dev. 18:362. 2. Wang LT, et al. 2012. Kaohsiung J. Med. Sci. 28:355. 3. Cornett B, et al. 2013. Dev. Biol. 2013. 379:38. 4. Das S, et al. 2012. Front. Biol. (Beijing). 7:587. 5. Stefater JA 3rd, et al. 2013. Blood 121:2574.
Gene ID: 79971
UniProt: View information about Wntless on UniProt.org
Clone: YJ5
Regulatory Status: RUO
Other Names: G Protein-Coupled Receptor 177 (GPR177), WLS, Protein Wntless Homolog, Putative NF-Kappa-B-Activating Protein 373, C1orf139, Evi
Isotype: Mouse IgG1, κ