Biolegend, 656408, PE/Cyanine7 anti-Th-POK (ZFP-67) Antibody, 100tests

Th-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4+ helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8+ T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4+ T cell lineage. Inactivation of Th-POK in mature CD4+ T cells results in induction of the genes preferentially expressed in CD8+ T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4+ T helper cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human Th-POK recombinant protein (197-345 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.This clone displays a similar affinity to BioLegend clone W15147A by western blot. However, this clone does not recognize mouse TH-POK.
RRID: AB_2927978 (BioLegend Cat. No. 656407) AB_2927978 (BioLegend Cat. No. 656408)
Structure: 539 amino acids, predicted molecular weight of 58 kD; contains four C2H2-type zinc finger regions that are responsible for DNA binding; contains a BTB-POZ domain which is involved in homodimerization and association with other factors
Distribution: Nucleus
Function: Transcription factor that regulates CD4 lineage commitment of immature T-cell precursors; acts as a transcriptional repressor of collagen and fibronectin genes
Interaction: Acetyltransferase p300
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: TCRs
Antigen References: 1. Kappes DJ. 2010. Immunol. Rev. 238:182. 2. Mariani F, et al. 2013. PLoS One. 8:e54488. 3. Zhang M, et al. 2010. J. Immunol. 185:3960. 4. Egawa T, et al. 2008. Nat. Immunol. 9:1131. 5. Wildt KF, et al. 2007. J. Immunol. 179:4405. 6. Setoguchi R, et al. 2009. J. Immunol. 183:4467.
Gene ID: 51043
UniProt: View information about Th-POK on UniProt.org
Clone: 11H11A14
Regulatory Status: RUO
Other Names: T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 7B (ZBTB7B), Krueppel-related zinc finger protein (cKrox), Zinc finger protein 67 homolog (ZFP-67)
Isotype: Mouse IgG1, κ