Biolegend, 658701, Purified anti-Bcl-2 Antibody, 25μg

Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Pezzella F, et al. 1993. New Eng. J. Med. 329:690. (IHC) Pezzella F, et al. 1990. Am. J. Pathol. 137:225. (IHC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Okimoto T, et al. 2020. Cancer Sci. 111:1910. PubMed Fischer JR, et al. 2023. Cell Rep Med. 4:100977. PubMed Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed Kartika ID, et al. 2021. Cancer Med. 10:8988. PubMed Wang F, et al. 2020. Int J Oncol. 57:707. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Chong SJF, et al. 2020. Nucleic Acids Res. 48:12727. PubMed Zhou X, et al. 2019. Med Sci Monit. 25:836. PubMed Jiang Q, et al. 2022. Stem Cells Int. 2022:5897194. PubMed Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed Inao T, et al. 2019. Cancer Sci. 110:2690. PubMed Lu IT, et al. 2022. Mar Drugs. 20:. PubMed
RRID: AB_2562958 (BioLegend Cat. No. 658701) AB_2562959 (BioLegend Cat. No. 658702)
Structure: Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
Distribution: Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
Function: Regulates apoptosis through controlling mitochondrial fusion and fission.
Interaction: BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References: 1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243. 2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7. 3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414. 4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571. 5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852.
Gene ID: 596
UniProt: View information about Bcl-2 on UniProt.org
Clone: 100
Regulatory Status: RUO
Other Names: B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50
Isotype: Mouse IgG1