Biolegend, 658901, Purified anti-Thioredoxin 1 Antibody, 25μg

Thioredoxin is an 11.7 kD E. coli cytoplasmic protein that act as an anti-oxidant in the cells. When used as a fusion partner to produce recombinant protein, thioredoxin has been shown to increase protein solubility and yield, as well as reduce protein aggregation and precipitation.
25μg
Verified Reactivity: E. coli
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length thioredoxin recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 4H12A59 anti-thioredoxin antibody can be used for Western blotting to detect E. coli thioredoxin-1 and thioredoxin tagged recombinant protein.
Product Citations: Sinha D, et al. 2020. Anal Biochem. 113827:608. PubMed
RRID: AB_2783364 (BioLegend Cat. No. 658901) AB_2562961 (BioLegend Cat. No. 658902)
Structure: 109 amino acids, predicted molecular weight of 12 kD.
Biology Area: Cell Biology
Antigen References: 1. LaVallie ER, et al. 2003. Methods Mol. Biol. 205:119. 2. Lu Z, et al. 1996. J. Biol. Chem. 271:5059. 3. LaVallie ER, et al. 1993. Biotechnology. 11:187.
Gene ID: 948289
UniProt: View information about Thioredoxin-1 on UniProt.org
Clone: 4H12A59
Regulatory Status: RUO
Other Names: Thioredoxin-1 (Trx1), Fip, FipA, TsnC, TrxA.
Isotype: Mouse IgG1, κ