Biolegend, 659002, Purified anti-TdT Antibody, 100μg

Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human TdT recombinant protein (1-508aa) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 7H9A33 clone has been shown to be useful for western blotting of human and mouse TdT.
RRID: AB_2562962 (BioLegend Cat. No. 659002)
Structure: 510 amino acids, predicted molecular weight of 58 kD.
Distribution: Nucleus.
Function: Template-independent DNA polymerase catalyzing the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. It is also involved in the addition of nucleotides at the N region junction of rearranged immunoglobulin heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Interaction: Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone.
Cell Type: B cells
Biology Area: Cell Biology, DNA Repair/Replication, Immunology
Molecular Family: Enzymes and Regulators
Antigen References: 1. Yunk L, et al. 2009. J. Immunol. 183:452. 2. Kedzierska K, et al. 2008. J. Immunol. 181:2556. 3. Fuller SA, et al. 1985. Biochem. J. 231:105. 4. Feeney AJ, et al. 2001. J. Immunol. 167:3486. 5. Komori T, et al. 1993. Science 261:1171.
Gene ID: 1791
UniProt: View information about TdT on UniProt.org
Clone: 7H9A33
Regulatory Status: RUO
Other Names: DNA nucleotidylexotransferase (DNTT), Terminal addition enzyme, Terminal transferase
Isotype: Mouse IgG1, κ