Biolegend, 659402, Purified anti-TICAM-2 (TRAM) Antibody, 100μg

TICAM-2, also known as TRIF-related adaptor molecule (TRAM), is a TIR domain containing protein that was found to act as an adaptor molecule during LPS-induced innate immune response. Upon infection by Gram-negative bacteria, TLR4 specifically recognizes LPS and leads to the dimerization of the cytoplasmic TIR domains. The activated TLR4 subsequently recruits the adaptor protein complex containing both TICAM-1 and TICAM-2, resulting in the activation of IFN response factors and production of pro-inflammatory cytokines. In this signaling cascade, TICAM-2 is essential for bridging the interaction between TLR4 and TICAM-1. TICAM-2 is also involved in the regulation of the IL-18 signaling pathway, acting as a sorting adaptor that recruits MYD88 from the cytosol to the membrane region upon activation of IL-1/IL-18 receptors.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human TICAM-2 recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Daudi cells were selected as a negative control for antibody testing due to the cell line’s low expression level of the mRNA encoding TICAM2 (source: Human Protein Atlas).
Product Citations: Holze J, et al. 2024. Nat Commun. 9554:15. PubMed
RRID: AB_2563252 (BioLegend Cat. No. 659402)
Structure: 235 amino acids encode a protein with a predicted MW of 27 kD. Contains a TIR domain responsible for mediating the physical interaction with TLR4 and TICAM-1.
Distribution: Cytoplasm, golgi apparatus, endosome, cell membrane, and endoplasmic reticulum.
Function: TICAM-2 acts as a sorting adaptor molecule that transduces TLR4 signaling to TICAM-1 and regulates MYD88-independent pathway in response to LPS stimulation.
Interaction: Interacts with TRAF6, MYD88, TLR4, IL1R1, IL1RAP, IRAK2, IRAK3, TICAM1, IRF3, and IRF7.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Antigen References: 1. Enokizono Y, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:19908. 2. Ohnishi H, et al. 2012. PLoS One. 7:e38423. 3. Wu B, et al. 2011. Dev. Comp. Immunol. 35:764. 4. Seya T, et al. 2005. Int. J. Biochem. Cell Biol. 37:524. 5. Vogel SN, et al. 2003. Mol. Interv. 3:466. 6. Oshiumi H, et al. 2003. J. Biol. Chem. 278:49751.
Gene ID: 353376
UniProt: View information about TICAM-2 on UniProt.org
Clone: 9G1B30
Regulatory Status: RUO
Other Names: TIR domain-containing adapter molecule 2, TRIF-related adapter molecule (TRAM), Toll-like receptor adaptor protein 3, Toll/interleukin-1 receptor domain-containing protein
Isotype: Mouse IgG2a, κ