Biolegend, 660552, Purified anti-CDA Antibody, 100μg

CDA, Cytidine deaminase, is an enzyme that plays a critical role in nucleic acid metabolism by catalyzing the deamination of cytidine to uridine. This reaction involves the removal of an amine group from cytidine, converting it into uridine and releasing ammonia as a byproduct. The importance of CDA lies in its involvement in several essential biological processes, including RNA editing, DNA repair, and pyrimidine metabolism. CDA is integral to the catabolic pathway of pyrimidine nucleotides, particularly the salvage pathway, which recycles nucleotides for DNA and RNA synthesis. Cytidine deaminase breaks down cytidine and deoxycytidine into uridine and deoxyuridine, respectively. These products can then be further processed to form bases and nucleosides for the maintenance of nucleotide pools. By regulating pyrimidine homeostasis, CDA ensures that cells have a balanced supply of nucleotides, which is essential for DNA replication, transcription, and overall cell proliferation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human CDA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: It is not recommended to use this product for Immunohistochemistry (IHC), immunocytochemistry (ICC), or intracellular flow cytometry (ICFC). This antibody is not recommended for use in Western blotting (WB) with mouse samples due to the presence of an unidentified band around 10 kDa.
RRID: AB_3675186 (BioLegend Cat. No. 660551) AB_3675186 (BioLegend Cat. No. 660552)
Structure: Homotetramer
Distribution: Expressed in lymphoid organs, liver, and esophagus. Located in the cytoplasm
Function: Scavenges cytidine and 2'-deoxycytidine for UMP synthesis
Biology Area: Cell Biology, Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family: Enzymes and Regulators
Antigen References: Schröder J K, et al. 1998. Br J Haematol. 103:1096-103. Bardenheuer W, et al. 2005. Leukemia. 19:2281-8. Mameri H, et al. 2017. Clin Cancer Res. 23:2116-2126.
Gene ID: 978
UniProt: View information about CD4 on UniProt.org
Clone: W20116C
Regulatory Status: RUO
Other Names: CDA, Cytidine Deaminase, Cytidine Aminohydrolase, Cytosine Nucleoside Deaminase, Small Cytidine Deaminase
Isotype: Rat IgG1, κ