Biolegend, 660702, Purified anti-IRF9 Antibody, 100μg

IRF9 is a member of the IRF family of transcription factors and plays an important role in IFN signaling pathways and anti-viral immune responses. Type I and type III interferon stimulation induces phosphorylation and heterodimerization of STAT1 and STAT2. The activated STAT1:STAT2 heterodimer recruits IRF9 and forms a heterotrimeric complex termed IFN-stimulated gene factor 3 (ISGF3). The ISGF3 complex, in turn, translocates to the nucleus and transactivates IFN-inducible genes. The IRF9 protein in the ISGF3 complex is responsible for the recognition of IFN-stimulated response elements (ISRE). Mice lacking IRF9 fail to induce the expression of IFN regulated genes in response to type I interferon stimulation, indicating that IRF9 is crucial in mediating interferon signaling.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human IRF9 recombinant protein (116-387 a.a.) expressed in E. coli.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Affinity purified.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 - 2.5 µg per mL. For ChIP applications, the suggested dilution is 1:50 to 1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not react with mouse (in-house tested). A non-specific band was observed (around 37 KD) when this antibody was tested on Raw264.7 cells
Product Citations: Xiang Q, et al. 2022. PLoS Pathog. 18:e1010676. PubMed
RRID: AB_2563575 (BioLegend Cat. No. 660702)
Structure: 393 amino acids, predicted molecular weight of 44 kD; contains a IRF tryptophan pentad repeat responsible for DNA binding.
Distribution: Cytoplasm, phosphorylated form translocated to the nucleus.
Function: IRF9 is a transcription factor that mediates interferon signaling and activates the expression of interferon inducible genes.
Interaction: Forms the interferon-stimulated gene factor 3 complex (ISGF3) with Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 in response to type I IFN stimulation.
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Fink K and Grandvaux N, 2013. JAKSTAT. 2:e27521. 2. Tsuno T, et al. 2009. J. Immunother. 32:803. 3. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417. 4. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033. 5. Xiao W, et al. 2001. J. Biol. Chem. 276:23275. 6. Veals SA, et al. 1992. Mol. Cell. Biol. 12:3315.
Gene ID: 10379
UniProt: View information about IRF9 on UniProt.org
Clone: 5A3A39
Regulatory Status: RUO
Other Names: Interferon regulatory factor 9 (IRF-9), Interferon-stimulated gene factor 3 gamma (ISGF-3 gamma), ISGF3 p48 subunit
Isotype: Mouse IgG2b, κ