Biolegend, 660851, Purified anti-AMD1 Antibody, 25μg

AMD1 (Adenosylmethionine Decarboxylase 1) is an enzyme critical for polyamine biosynthesis, a process vital for cell growth, proliferation, and differentiation. It decarboxylates S-adenosylmethionine (SAM) to form decarboxylated SAM (dcSAM), a crucial step in the synthesis of spermidine and spermine, which plays a key role in stabilizing cellular structures like DNA and RNA. AMD1 maintains normal cell function through polyamine regulation, while its dysregulation is associated with cancer, neurological diseases, and inflammation. In many cancers, such as colon, prostate, and breast cancer, AMD1 is overexpressed. This leads to elevated polyamine levels, promoting rapid cell division, uncontrolled growth, and tumor progression. Given its role in driving tumor growth, AMD1 is viewed as a potential target for cancer therapy. Inhibiting AMD1 may decrease polyamine production, thereby slowing cancer progression.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human AMD1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). It is not recommended using this product in immunocytochemistry (ICC) or intracellular flow cytometry (ICFC). This antibody does not cross react with mouse AMD1 in Western Blot.
RRID: AB_3675187 (BioLegend Cat. No. 660851) AB_3675187 (BioLegend Cat. No. 660852)
Structure: Heterotetramer of two alpha and two beta chains; AMD1 is a 334 amino acid length protein with a predicted molecular weight of 38.3 kD.
Distribution: Widely expressed in tissues. Located in the cytoplasm
Function: Essential for biosynthesis of the polyamines spermidine and spermine
Biology Area: Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family: Enzymes and Regulators
Gene ID: 262
UniProt: View information about AMD1 on UniProt.org
Clone: W18041A
Regulatory Status: RUO
Other Names: Adenosylmethionine Decarboxylase 1, SAMDC, S-Adenosylmethionine Decarboxylase 1, S-Adenosylmethionine Decarboxylase Proenzyme, AdoMetDC
Isotype: Rat IgG2b, κ