Biolegend, 661302, Purified anti-DDX5 Antibody, 100μg

DDX5, also named RNA helicase p68, is a prototypic member of the DEAD (Asp-Glu-Ala-Asp) box family. DDX5 has been shown to be involved in the processes requiring the alteration of RNA structures, such as the maturation of pre-mRNA, rRNA, and miRNA. DDX5 also plays important helicase-independent roles in transcriptional regulation. DDX5 can act as a transcriptional coactivator of the androgen receptor (AR), estrogen receptor α (ERα), tumor suppressor p53, MyoD, RUNX2, and β-catenin, which are involved in the regulation of cell proliferation, differentiation, and tumorigenesis. DDX5 has been shown to be up-regulated in various cancers, including breast cancer, prostate cancer, and colorectal cancer, suggesting that DDX5 may participate in tumor development. DDX5 can also act as a transcriptional repressor through interacting with HDAC1.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human DDX5 recombinant protein (482-614 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunocytochemistry, the suggested use of this reagent is 2.0 - 10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2563942 (BioLegend Cat. No. 661302)
Structure: 614 amino acids, predicted molecular weight of 69 kD. Contains a DEAD box domain, a helicase domain, and a transactivation domain.
Distribution: Nucleus.
Function: DDX5 is an ATP-dependent RNA helicase, regulating RNA processing and ribosome biogenesis. DDX5 is also involved in transcription by acting as a transcriptional coactivator or a repressor.
Interaction: DDX5 interacts with DDX17, RBM4, AGO1, AGO2, ESR1, p300, RUNX2, and HDAC1.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Antigen References: 1. Iyer RS, et al. 2014. Cell Cycle. 13:1413. 2. Samaan S, et al. 2014. Nucleic Acids Res. 42:2197. 3. Fuller-Pace FV. 2013. Biochim. Biophys. Acta. 1829:756. 4. Wang D, et al. 2012. Mol. Cell Proteomics. 11:M111.011932. 5. Saporita AJ, et al. 2011. Cancer Res. 71:6708. 6. Clark EL, et al. 2008. Cancer Res. 68:7938.
Gene ID: 1655
UniProt: View information about DDX5 on UniProt.org
Clone: 14B9A58
Regulatory Status: RUO
Other Names: Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68
Isotype: Mouse IgG2b, κ