Biolegend, 662602, Purified anti-IL-27/IL-35 EBI3 (monomer, dimer, heterodimer) Antibody, 100μg

EBI3 is an IL-12 p40-related protein that can heterodimerized with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells (DCs) following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 differentiation, increase cytolytic activity of CD8 + T cells, and impact B cell differentiation and immunoglobulin production. IL-35 is also involved in tumorigenesis and is a promising biomarker for assessing tumor progression and prognosis in clinical settings.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse EBI3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2564303 (BioLegend Cat. No. 662602)
Structure: 229 amino acids, predicted molecular weight of approximately 25 kD.
Distribution: B cells, monocytes, and dendritic cells.
Function: Pro- and anti-inflammatory.
Interaction: IL-27 p28 subunit and IL-35 p35 subunit.
Ligand/Receptor: WSX-1(TCCR) and gp130.
Cell Type: B cells, Dendritic cells, Monocytes, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Pflanz S, et al. 2002. Immunity 16:779. 2. Takeda A, et al. 2003. J. Immunol. 170:4886. 3. Pflanz S, et al. 2004. J. Immunol. 172:2225. 4. Li MF, et al. 2013. Dev. Comp. Immunol. 41:514. 5. Zeng JC, et al. 2013. Int. J. Clin. Exp. Pathol. 6:1806. 6. Ouyang H, et al. 2014. Am. J. Med. Sci. 348:156.
Gene ID: 10148
UniProt: View information about IL-27 IL-35 on UniProt.org
Clone: J053B5
Regulatory Status: RUO
Other Names: Epstein-Barr Virus Induced 3, EBV-Induced Gene 3 Protein, IL-27 Subunit Beta, IL-27B, IL-35 subunit
Isotype: Rat IgG2a, κ