Biolegend, 663205, Direct-Blot™ HRP anti-, 100microl

RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is a part of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion.
100microl
Verified Reactivity: E. coli
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486. Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Breyer MJ, et al. 1997. J. Bacterial 179:1404. Lesley SA, et al. 1989. J. Biol. Chem. 264:3794. Strickland MS, et al. 1988. Biochemistry 27:5755.
Product Citations: Carobbi A, et al. 2022. Environ Microbiol. 24:4787. PubMed Hughes KJ, et al. 2020. Cell Rep. 33:108527. PubMed Piel D, et al. 2020. Environ Microbiol. 4198:22. PubMed Adams DW, et al. 2019. Nat Microbiol. 1.239583333. PubMed Dar Y, et al. 2022. EMBO Rep. 23:e53981. PubMed Yang H, et al. 2021. Cell Discov. 7:90. PubMed
RRID: AB_2629596 (BioLegend Cat. No. 663205)
Structure: Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 947567
UniProt: View information about Sigma 70 on UniProt.org
Clone: 2G10
Regulatory Status: RUO
Other Names: RNA pol sigma 70, rpoD, sigma D, RNA Polymerase
Isotype: Mouse IgG3, κ