Biolegend, 663404, Purified anti-, 25μg

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters.
25μg
Verified Reactivity: E. coli
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 3RH3 recognizes epitope 1-25. Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Lesley SA, et al. 1987. J. Biol. Chem. 262:5404.
RRID: AB_2750247 (BioLegend Cat. No. 663404) AB_2750248 (BioLegend Cat. No. 663405)
Structure: Sigma 32 (σ32) is a 32 kD transcription factor of E. coli RNA polymerase.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 947970
UniProt: View information about Sigma 32 on UniProt.org
Clone: 3RH3
Regulatory Status: RUO
Other Names: SigmaH, rpoH, fam, hin, htpR, Heat shock regulatory protein F33.4, RNA Polymerase
Isotype: Mouse IgG1