Biolegend, 663905, Purified anti-, 100μg

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.
100μg
Verified Reactivity: E. coli
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant E. coli RNA polymerase β subunit.
Formulation: 0.22 µm filtered protein solution is in phosphate-buffered solution, pH 7.2.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested ELISA, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.
Application References(PubMed link indicates BioLegend citation): Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
Product Citations: Cui Y, et al. 2022. Appl Environ Microbiol. 88:e0130522. PubMed Wong AI, et al. 2023. Elife. 12:. PubMed Dupuy P, et al. 2023. Elife. 12:. PubMed Tang MX, et al. 2022. ISME J. 16:1765. PubMed Morreale FE, et al. 2022. Cell. 185:2338. PubMed Chihara K, et al. 2022. RNA. :. PubMed d'Andrea FB, et al. 2022. Sci Adv. 8:eabn7943. PubMed Dupuy P, et al. 2020. Proc Natl Acad Sci U S A. 117:19517. PubMed Wipperman MF et al. 2018. Molecular cell. 72(1):152-161 . PubMed Olivencia B,et al. 2017. Sci Rep.. 10.1038/s41598-017-14410-z. PubMed Liang X, et al. 2021. PLoS Pathog. 17:e1010116. PubMed Hör J, et al. 2020. Nucleic Acids Res. 48:9301. PubMed Dupuy P, et al. 2022. Nat Commun. 13:4493. PubMed Liang X, et al. 2021. Appl Environ Microbiol. 87:e0043521. PubMed
RRID: AB_2566583 (BioLegend Cat. No. 663905)
Structure: 1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution: Bacteria.
Function: Transcriptional regulator in bacteria.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Thompson NE, et al. 1992. Biochemistry 31:7003. 2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477. 3. Hirata A, et al. 2008. Nature. 451:851. 4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155. 5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
Gene ID: 948488
UniProt: View information about RNA Polymerase beta on UniProt.org
Clone: 8RB13
Regulatory Status: RUO
Other Names: RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
Isotype: Mouse IgG2b, κ