Biolegend, 664304, Purified anti-, 100μg

CRP (also known as cAMP receptor protein) is a regulatory protein in bacteria. CRP binding to cAMP leads to conformational changes in CRP resulting in a dramatic increase in its binding to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA and acts as a negative regulator of its own synthesis.
100μg
Verified Reactivity: E. coli
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified protein
Formulation: Phosphate-buffered solution, pH 7.2.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Huang Y, et al. 2022. Gut Microbes. 14:2136460. PubMed Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed Lauritsen I, et al. 2021. Nat Commun. 12:5880. PubMed Ayhan D, et al. 2016. PLoS Biol. 14: 1002552. PubMed Seok JY, et al. 2021. Cell Reports. 36(8):109589. PubMed Pryor R et al. 2019. Cell. 178(6):1299-1312 . PubMed
RRID: AB_2565553 (BioLegend Cat. No. 664304)
Structure: 210 amino acids with a predicted molecular weight of approximately 23.5 kD.
Distribution: Bacteria.
Function: Transcriptional regulator in bacteria.
Ligand/Receptor: cAMP
Biology Area: Cell Biology, Transcription Factors
Antigen References: 1. Busby S, et al. 1999. J. Mol. Biol. 293:199. 2. Lawson CL, et al. 2004. Curr. Opin. Struct. Biol. 14:10. 3. Hudson BP, et al. 2009. Proc. Natl. Acad. Sci. USA 106:19830.
Gene ID: 1036667
UniProt: View information about CRP on UniProt.org
Clone: 1D8D9
Regulatory Status: RUO
Other Names: cAMP receptor protein, cAMP regulatory protein, Catabolite gene activator, cap, csm
Isotype: Mouse IgG1