Biolegend, 664801, Purified anti-β-actin Antibody, 25μg

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
25μg
Verified Reactivity: Human, Mouse, Rat, Zebrafish, Drosophila
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human ACTB peptide (1-13 a.a.).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg/ml in human, mouse, rat and zebrafish and 0.1 - 0.5 µg/ml in Drosophila. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Reactivity to Drosophila was only verified with the purified format.
Product Citations: Wang L, et al. 2022. Viruses. 14: . PubMed Park SJ, et al. 2023. Int J Mol Sci. 24: . PubMed Lippert TP, et al. 2021. Nat Commun. 0.855555556. PubMed Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed Balcioglu O, et al. 2020. Breast Cancer Res. 1.003472222. PubMed Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
RRID: AB_2721350 (BioLegend Cat. No. 664801) AB_2721349 (BioLegend Cat. No. 664802)
Structure: 375 amino acids with a predicted molecular weight of 42 kD.
Distribution: Cytoplasm
Function: Actins are highly conserved proteins that are involved in cell motility.
Interaction: Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Antigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
Gene ID: 60
UniProt: View information about beta-actin on UniProt.org
Clone: W16197A
Regulatory Status: RUO
Other Names: ACTB, Actin, cytoplasmic 1, Beta-actin
Isotype: Rat IgG2a, κ