Biolegend, 665853, Ultra-LEAF™ Purified anti-human CCL1 (I-309) Antibody, 100μg

CCL1 was initially identified as a secreted protein derived from activated T cells. CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation. As a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and, compared to normal patients, higher concentration of CCL1 is observed in bronchoalveolar lavage of asthmatic patients. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication demonstrated that in in-vitro conditions, CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β. Furthermore, this transcriptional upregulation is reversed could be reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Also, antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human CCL1 (I-309)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the chemotaxis of mouse Baf3- hCCR8 cells induced by recombinant human CCL1 (I-309) (Cat. No. 582702) at 0.6 ng/mL. ND50 range: 0. 1- 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3717089 (BioLegend Cat. No. 665853) AB_3717089 (BioLegend Cat. No. 665854)
Structure: Chemokine
Distribution: T cells, Th1, Th2, diabetogenic T cells, peritoneal macrophages, peritoneal mesothelial cells, Langerhans cells, monocytes, mast cells, NK cells, endothelial cells, and epithelial cells
Function: CCL1 chemoattracts CD4 Th2 cells and plays a key role in the progression of Th2 type hypersensitivity.
Interaction: T cells, Th2 cells, macrophages, microglia, FOXP3+ CD4 regulatory T cells, skin-homing CLA+T cells, monocytes, NK cells, dendritic cells, endothelial cells, and smooth muscle cells
Ligand/Receptor: CCR8 (CDw198)
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Miller MD, et al. 1989. J Immunol. 143:2907-16. Haque NS, et al. 2004. Blood. 103:1296-304 Gombert M, et al. 2005. J Immunol. 174:5082-91. Montes-Vizuet R, et al. 2006. Eur Respir J. 28:59-67. Soler D, et al. 2006. J Immunol. 177:6940-51. Gonzalo JA, et al. 2007. J Immunol. 179:1740-50. Mutalithas K, et al. 2010. Clin Exp Allergy. 40:1175-85.
Gene ID: 6346
UniProt: View information about CCL1 on UniProt.org
Clone: A16001D
Regulatory Status: RUO
Other Names: T lymphocyte-secreted protein I-309
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.