Biolegend, 666401, Purified anti-STAT1 Phospho (Tyr701) Antibody, 25μg

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated at Tyrosine 701 (Tyr701) by receptor-associated kinases, including JAK1, JAK2, and TYK2. This results in STAT1 dimerization and subsequent translocation to the nucleus, where it functions as a transcriptional activator. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: WB - Quality tested ICFC, ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml (1:250-5000 dilution). For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunocytochemistry, a range of 5.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50 by volume. It is recommended that the reagent be titrated for optimal performance for each application. For intracellular flow cytometry our True-Nuclear™ Transcription Factor Staining Protocol is recommended.
Application Notes: Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701). When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.
RRID: AB_2728499 (BioLegend Cat. No. 666401) AB_2728500 (BioLegend Cat. No. 666402)
Structure: Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Distribution: Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution
Function: Immune response activation, cell signaling
Interaction: STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
Biology Area: Cell Biology
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Moretti S, et al. 2017. J. Biol. Chem. 292: 1785. Wei J, et al. 2015. J. Immunol. 195: 2870. Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443 Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909. Wu TR, et al. 2002. J. Biol. Chem. 277: 47572. Horvath, et al. 1996. J. Virol. 70: 647. Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
Gene ID: 6772
UniProt: View information about STAT1 Phospho Tyr701 on UniProt.org
Clone: A17012A
Regulatory Status: RUO
Other Names: Signal Transduced Activator of Transcription 1, Transcription Factor ISGF-3 components p91/p84
Isotype: Mouse IgG1, κ