Biolegend, 669801, Purified anti-Skp1 Antibody, 25μg

S-phase kinase-associated protein 1 (Skp1) is one of the components of the SCF complex, which belongs to the E3 ubiquitin-protein ligase family. The SCF complex recruits substrates and promotes ubiquitin-mediated degradation of the substrates. Skp1 forms an invariable core component of the SCF complex with Cul1 and Rbx1 proteins. Skp1 binds to F-box proteins, which recruit the substrates for proteolysis.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length recombinant human Skp1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes human Skp1 and cross-reacts with mouse Skp1.When tested for WB, this clone detected a ~40 kDa protein of unknown origin; this band was not observed at lower antibody titrations.Because of the binding of this antibody to the unknown protein, this clone was not tested for ICC.This clone failed IP testing.
RRID: AB_2820110 (BioLegend Cat. No. 669801) AB_2820111 (BioLegend Cat. No. 669802)
Structure: Skp1 is a 163 amino acid protein with a predicted molecular weight of 19 kD.
Distribution: Centrosome/Cytosol/Nucleus/Ubiquitously expressed
Function: Adaptor protein
Biology Area: Cell Biology, Ubiquitin/Protein Degradation
Molecular Family: Adaptor Proteins
Antigen References: Bai C, et al. 1996. Cell. 86:263 Zheng N, et al. 2002. Nature. 416:703 Schulman B, et al. 2000. Nature. 408:381
Gene ID: 6500
UniProt: View information about Skp1 on UniProt.org
Clone: W18108A
Regulatory Status: RUO
Other Names: S-Phase Kinase Associated Protein 1 , Transcription Elongation Factor B Polypeptide 1-Like, Cyclin-A/CDK2-Associated Protein P19, Organ Of Corti Protein II, P19skp1, OCP-II, TCEB1L, SKP1A, EMC19, SIII,RNA Polymerase II Elongation Factor-Like Protein
Isotype: Rat IgG2a, κ