Biolegend, 670002, Purified anti-Cyclophilin D Antibody, 100μg

Cyclophilin D is one of the three components of the permeability transition pore (PTP) localized in the mitochondrial matrix. The opening of the PTP is a key event in the initiation of cell death which leads to mitochondrial swelling, outer membrane rupture, and the release of apoptotic regulators such as cytochrome C. Cyclophilin D promotes mitochondrial permeability transition (mPT) in response to increased Ca 2+ level. Cyclophilin D deficient mice have shown desensitization to mTP and require twice as much Ca 2+ intake for the opening of the PTP compared to wild-type mice.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human PPIF protein (30-207 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes human cyclophilin D This clone failed IP testing
Product Citations: Kim S, et al. 2022. Cell Stress Chaperones. 27:573. PubMed
RRID: AB_2814520 (BioLegend Cat. No. 670001) AB_2814521 (BioLegend Cat. No. 670002)
Structure: Cyclophilin D is a 207 amino acid protein with a predicted molecular weight of 22 kD
Distribution: Mitochondrial matrix/Ubiquitously expressed
Function: Apoptotic regulator
Biology Area: Cell Death, Mitochondrial Function
Molecular Family: Mitochondrial Markers
Antigen References: Baines C, et al. 2005. Nature. 434:658 Schinzel A, et al. 2005. PNAS. 102:12005 Basso E, et al. 2005. J BioL Chem. 280:18558
Gene ID: 10105
UniProt: View information about Cyclophilin D on UniProt.org
Clone: W17239A
Regulatory Status: RUO
Other Names: Peptidylprolyl Isomerase F, Peptidyl-Prolyl Cis-Trans Isomerase F, Mitochondrial, Mitochondrial Cyclophilin, Cyclophilin F, Rotamase F, PPIase F, CyP-M, CYP3, CypD, Cyclophilin 3, Cyp-D, CyP-D, PPIF
Isotype: Rat IgG2a, λ