Biolegend, 670802, Purified anti-EML4 Antibody, 100μg

Echinoderm microtubule-associated protein-like 4 or EML4 plays a central role in the modification of microtubule structural dynamics and morphology. During mitosis, EML4 expression is upregulated and is associated with spindle formation. In lung cancer cells, a translocation mutation is associated with the EML4 gene, where parts of the gene fuses with the anaplastic lymphoma receptor tyrosine kinase ( ALK ) gene and generates an EML4-ALK fusion protein.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human EML4 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg/mL. For immunocytochemistry, a concentration of 1.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has not been tested for IP.
RRID: AB_2814524 (BioLegend Cat. No. 670801) AB_2814525 (BioLegend Cat. No. 670802)
Structure: EML4 is a 981 amino acid protein with a predicted molecular weight of 109 kD
Distribution: Cytoskeleton/Ubiquitously expressed
Function: Microtubule formation
Antigen References: Yoshida A, et al. 2011. J Thorac Oncol. 10:1677-86. Soda M, et al. 2007. Nature. 448:561. Pollmann M, et al. 2006. Exp Cell Res. (17):3241-51.
Gene ID: 27436
UniProt: View information about EML4 on UniProt.org
Clone: W18139A
Regulatory Status: RUO
Other Names: EML4, Echinoderm microtubule-associated protein-like 4
Isotype: Rat IgG2b, κ