Biolegend, 671201, Purified anti-PKCι Antibody, 25μg

Members of the protein kinase c family (PKC) are serine/threonine protein kinases with a broad set of substrates that can regulate diverse cellular processes. PKCι, also referred to as PKCι/λ, is a member of the atypical subfamily of PKC, which is characterized by complete independence of calcium and diacylglycerol as second messengers. It has been shown to play a role in cancer survival through multiple mechanisms: a. phosphorylation of the DNA-binding domain of FoxO1 resulting in transcriptional reprogramming of c-myc, which leads to increased proliferation of angiosarcoma cells, b. regulating membrane trafficking of type 1-matrix metalloprotease from late endosomal compartments to the plasma membrane, and c. mediating pro-survival by phosphorylating and inhibiting the pro-apoptotic factor BAD in glioblastomas. PKCι is also required for resistance to anti-apoptotic drugs in BCR-Abl-dependent leukemia and may represent a viable target for combinatorial therapies.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human PKCι protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with methanol or Triton X-100; it failed due to weak staining.
RRID: AB_2820114 (BioLegend Cat. No. 671201) AB_2820115 (BioLegend Cat. No. 671202)
Structure: PKCι is a 596 amino acid protein with a predicted molecular weight of 68 kD.
Distribution: Cytosol and Nucleus/Ubiquitously expressed
Function: Protein Kinase/Cell Signaling
Biology Area: Cell Biology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Nayak RC, et al. 2019. Nat. Commun. 10:46. Riddell M, et al. 2018. Nat. Commun. 9:5357. Rosse C, et al. 2014. Proc Natl Acad Sci U S A. 111:E1872. Desai S, et al. 2011. Biochim Biophys Acta. 1813:1190. Ishiguro H, et al. 2009. Proc Natl Acad Sci U S A. 106:16369.
Gene ID: 5584
UniProt: View information about PKCiota on UniProt.org
Clone: W18205A
Regulatory Status: RUO
Other Names: Protein Kinase C iota, Atypical Protein Kinase C-lambda/iota, Protein Kinase C iota Type, PRKC-lambda/iota, aPKC-lambda/iota, atypical protein kinase C, aPKC, aPKCλ/ι
Isotype: Rat IgG2a, κ