Biolegend, 671854, Ultra-LEAF™ Purified anti-human CD86 Antibody, 1mg

CD86, also known as B7-2, is a member of the cell surface immunoglobulin superfamily. B7 family members are transmembrane molecules that exist predominantly as monomers. They are classified into three groups according to their functions. Group I members include B7-1, B7-2, and B7-H2. These members are either co-stimulatory or co-inhibitory in modulating T cell response. Group II members include B7-H1 (PD-L1) and B7-DC (PD-L2) which are ligands for PD-1. These members are involved in regulating induction and maintenance of peripheral immune tolerance. Group III members include B7-H3 and B7-H4 whose role in T cell regulation has yet to be defined. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human CD86
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.15 µg/mL recombinant biotinylated human CTLA-4-Fc (Cat. No. 786706) to immobilized 1 µg/mL recombinant human B7-2/CD86-Fc chimera (Cat. No. 771706). ND50 range: 0.015 - 0.15 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3699085 (BioLegend Cat. No. 671853) AB_3699085 (BioLegend Cat. No. 671854)
Structure: Immunoglobulin family
Distribution: Activated antigen presenting cells, including B cells, dendritic cells and macrophages
Function: B7-2 is a costimulatory molecule that is required for T cell proliferation and IL-2 production.
Interaction: Leukocytes, endothelial cells
Ligand/Receptor: CD28, CTLA4
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: Freeman GJ, et al. 1993. Science. 262:909-11. Chen C, et al. 1994. J Immunol. 152:4929-36. Lanier LL, et al. 1995. J Immunol. 154:97-105. Carreno BM, et al. 2002. Annu Rev Immunol. 20:29-53. Rudd CE, et al. 2009. Immunol Rev. 229:12-26. Wing K, et al. 2011. Trends Immunol. 32:428-33.
Gene ID: 942
UniProt: View information about CD86 on UniProt.org
Clone: W17233G
Regulatory Status: RUO
Other Names: CD86, B7-2, T lymphocyte activation antigen CD86, Activation B7-2 antigen, CD28 antigen ligand 2, CD28LG2B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.