Biolegend, 673152, Purified anti-STK4 Antibody, 100μg

Serine/Threonine Kinase 4, also known as STK4 or MST1, is essential for regulating cellular processes like stress response, apoptosis, and organ size control. Functionally, STK4 is a stress-activated kinase that can induce apoptosis by entering the nucleus and causing chromatin condensation and DNA fragmentation. It plays a crucial role in the Hippo signaling pathway, which restricts cell proliferation and promotes apoptosis, thereby preventing tumor growth. STK4 is regulated through its interactions with other proteins in a kinase cascade, including STK3/MST2 and LATS1/2, which further modulate proteins involved in cell growth and apoptosis. Mutations or deficiencies in STK4 can lead to severe immunodeficiencies and other health issues.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human STK4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody on immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend using Tris-EDTA buffer (Cat. No. 422704) for antigen retrieval. We do not recommend citrate buffer.
RRID: AB_3683494 (BioLegend Cat. No. 673151) AB_3683494 (BioLegend Cat. No. 673152)
Structure: STK4 is a 487 amino acid protein with a molecular weight of approximately 55 kDa.
Distribution: The STK4 protein is primarily localized in the cytoplasm but upon activation can be found in the nucleus
Function: The regulation of STK4 involves several mechanisms: - Phosphorylation: STK4 is activated through phosphorylation - Protein Interactions: STK4 interacts with various proteins in the Hippo signaling pathway, including STK3/MST2 and LATS1/2 - Stress Response: STK4 is activated in response to cellular stress - Negative Regulation: STK4 can negatively regulate certain pathways, such as the TBK1-IRF3 signaling pathway
Interaction: STK4 interacts with various proteins in the Hippo signaling pathway, including STK3/MST2 and LATS1/2.
Antigen References: Abdollahpour H, et al. 2012. Blood. 119:3450. Jorgensen SE, et al. 2020. J Clin Immunology. 41:109. Guennoun A, et al. 2021. J Clin Immunology. 41:1839. Nehme NT, et al. 2012. Blood. 119:3458.
Gene ID: 6789
UniProt: View information about STK4 on UniProt.org
Clone: W21145C
Regulatory Status: RUO
Other Names: MST1, KRS2, YSK3, MAmmalian STE20-Like protein kinase 1, Serine/Threonine-Protein Kinase Krs-2, Kinase responsive to stress 2
Isotype: Rat IgG2b, κ