Biolegend, 674001, Purified anti-Nanog Antibody, 25μg

Nanog is a transcription factor that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors, which include Sox2 and Oct-4. It also plays a role in epithelial malignancy and is enriched in cancer stem cells. The ablation of nanog is sufficient to reduce the cancer stem cell pool.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human recombinant protein expressed in bacteria
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted at 4°C
Application: WB - Quality tested ICFC, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is ≤ 0.175 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2564570 (BioLegend Cat. No. 674001) AB_2564571 (BioLegend Cat. No. 674002)
Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution: Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.
Function: Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.
Interaction: Interacts with SMAD1, SALL4, and ZNF281/ZFP281.
Cell Type: Embryonic Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors
Antigen References: 1. Hwang CC, et al. 2014. PLoS One 9:e105676. 2. Torres-Padilla ME, et al. 2014. Development 141:2173. 3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741. 4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207. 5. Kim J, et al. 2008. Cell 132:1049. 6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081. 7. Yu J, et al. 2007. Science 318:1917.
Gene ID: 79923
UniProt: View information about Nanog on UniProt.org
Clone: 23D2-3C6
Regulatory Status: RUO
Other Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype: Mouse IgG1, κ