Biolegend, 674010, Alexa Fluor® 647 anti-Nanog Antibody, 100μg

Nanog is a transcription factor that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors, which include Sox2 and Oct-4. It also plays a role in epithelial malignancy and is enriched in cancer stem cells. The ablation of nanog is sufficient to reduce the cancer stem cell pool.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human recombinant protein expressed in bacteria
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Chen Z, et al. 2024. J Extracell Vesicles. e12409:13. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 72.77986111. PubMed Ibuki M, et al. 2019. Regen Ther. 0.551388889. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed
RRID: AB_2632605 (BioLegend Cat. No. 674010)
Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution: Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.
Function: Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.
Interaction: Interacts with SMAD1, SALL4, and ZNF281/ZFP281.
Cell Type: Embryonic Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors
Antigen References: 1. Hwang CC, et al. 2014. PLoS One 9:e105676. 2. Torres-Padilla ME, et al. 2014. Development 141:2173. 3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741. 4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207. 5. Kim J, et al. 2008. Cell 132:1049. 6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081. 7. Yu J, et al. 2007. Science 318:1917.
Gene ID: 79923
UniProt: View information about Nanog on UniProt.org
Clone: 23D2-3C6
Regulatory Status: RUO
Other Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype: Mouse IgG1, κ