Biolegend, 674704, Alexa Fluor® 594 anti-LIF Antibody, 100μg

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including the thymus, lung, and neuronal tissue. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contributes to stem cell self-renewal. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, as well as the induction of neuronal cell differentiation. It also regulates mesenchymal to epithelial conversion during kidney development, and also may have a role in immune tolerance at the maternal-fetal interface. LIF can be upregulated by pro-inflammatory cytokines such as TNF-α and IL-17. Elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse LIF produced in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
RRID: AB_2566178 (BioLegend Cat. No. 674704)
Structure: 202 amino acids with a predicted molecular weight of approximately 22 kD.
Distribution: Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.
Function: Inhibits embryonic stem cell differentation and induces the terminal differentiation of myeloid leukemia cells. LIF is upregulated by TNF-α and IL-17. LIF is downregulated by IL-4 and IL-13 in fibroblasts.
Interaction: Embryonic stem cells.
Ligand/Receptor: LIF receptor complex (gp190 and gp130).
Cell Type: T cells, Tregs
Biology Area: Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Moreau JF, et al. 1988. Nature 336:690. 2. Tomida M, et al. FEBS Lett. 334:193. 3. Metcalfe SM. 2011. Genes Immun. 12:157. 4. Voyle RB, et al. 1999. Exp. Cell Res. 249:199. 5. Slaets H, et al. 2010. Trends Mol. Med. 16:493. 6. Souza PP, et al. 2012. Mol. Immunol. 49:601
Gene ID: 3976
UniProt: View information about LIF on UniProt.org
Clone: M1506B09
Regulatory Status: RUO
Other Names: Leukemia inhibitory factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
Isotype: Rat IgG2a, κ