Biolegend, 675002, Purified anti-Leptin Antibody, 100μg

Leptin is primarily synthesized from adipocytes and plays a major role in energy homeostasis and obesity. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, expression of the short form of the leptin receptor, Ob-Ra, is downregulated, and the individual may be unresponsive to leptin signals. Leptin has also been implicated in the regulation of reproduction, glucose homeostasis, bone formation, hematopoiesis, and wound healing.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Leptin.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Lee S, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID: AB_2565487 (BioLegend Cat. No. 675002)
Structure: 167 amino acids with a predicted molecular weight of approximately 18 kD.
Distribution: Secreted by adipocytes, gastric epithelial cells, or fibroblasts.
Function: Plays a major role in energy homeostasis and obesity.
Interaction: Interacts with Siglec-6.
Ligand/Receptor: Leptin receptors.
Cell Type: Epithelial cells, Fibroblasts
Biology Area: Angiogenesis, Cell Biology, Signal Transduction
Molecular Family: Enzymes and Regulators
Antigen References: 1. Ando S, et al. 2014. Front Oncol. 4:340. 2. Upadhyay J, et al. 2015. Metabolism 64:105. 3. Sainz N, et al. 2015. Metabolism 64:35. 4. Zhang F, et al. 1997. Nature 387:206. 5. Brennan AM, et al. 2006. Nat. Clin. Pract. Endocrinol. Metab. 2:318. 6. Halaas JL, et al. 1995. Science 269:543.
Gene ID: 3952
UniProt: View information about Leptin on UniProt.org
Clone: M1905E01
Regulatory Status: RUO
Other Names: Obese Protein, Obesity Factor, LEPD, OB, OBS
Isotype: Mouse IgG2a, λ