Biolegend, 675102, Purified anti-IL-34 Antibody, 100μg

IL-34 is a glycoprotein that plays important roles in the proliferation, survival, and differentiation of monocytes and macrophages. IL-34 is expressed in the heart, brain, lung, liver, kidney, thymus, spleen, and other organs. It specifically binds to CD115/M-CSF receptor (CSF-1 receptor or c-Fms) and activates ERK1/2 phosphorylation. The function of IL-34 overlaps with CSF-1 to regulate myeloid lineage development. IL-34 also promotes inflammatory processes and regulates osteoclast proliferation, differentiation, and bone reabsorption.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse IL-34 produced in CHO cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2 - 5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The theoretical MW of IL-34 is 27 kD, the observed MW is 50 kD.
RRID: AB_2565492 (BioLegend Cat. No. 675102)
Structure: 215 amino acids with a predicted molecular weight of approximately 26 kD.
Distribution: Expressed in different tissues including the spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostrate, and colon.
Function: Regulates myeloid lineage differentiation, proliferation, and survival.
Interaction: Macrophages, monocytes, and myeloid cells.
Ligand/Receptor: CD115/M-CSF receptor (CSF-1 receptor or c-Fms)
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lin H, et al. 2008. Sciences 320:807. 2. Chihara T, et al. 2010. Cell Death Differ. 17:1917. 3. Wei S, et al. 2010. J. Leuko. Biol. 88:495. 4. Eda H, et al. 2011. Rheumatol. 31:1525. 5. Covaleda L, et al. 2010. Virology 397:217.
Gene ID: 762577
UniProt: View information about IL-34 on UniProt.org
Clone: C051-77
Regulatory Status: RUO
Other Names: IL34, Interleukin 34, Chromosome 16 Open Reading Frame 77, C16orf77
Isotype: Rat IgG2c, κ