Biolegend, 675202, Purified anti-NLRP12 Antibody, 100μg

NLRP12, a member of the Nod-like receptor (NLR) family of proteins, was originally suggested to act as an inflammasome forming component and a modulator of NF-κB signaling. Recent studies revealed that NLRP12 plays anti-inflammatory roles, as NLRP12-deficient mice are more susceptible to chemically induced colitis, and show a more severe infiltration of granulocytes and T cells. The colitis induced in NLRP12 -/- mice is associated with increased production of proinflammatory cytokines and activation of both NF-κB and ERK signaling pathways. NLRP12 was reported to attenuate canonical NF-κB signaling by inhibiting IRAK-1 phosphorylation. NLRP12 also down-regulates non-canonical NF-κB signaling through interaction with TRAF3 and the NF-κB inducing kinase NIK.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human NLRP12 recombinant protein (93-1061 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2565493 (BioLegend Cat. No. 675202)
Structure: 1061 amino acids with a predicted molecular weight of 120 kD. Contains a DAPIN domain, a NACHT-associated domain, and eight leucine-rich repeats (LRR).
Distribution: Cytoplasm.
Function: NLRP12 negatively regulatres inflammation through attenuating both canonical and non-canonical NF-κB signaling.
Interaction: Interacts with ASC and FAF1 through its DAPIN domain.
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Allen IC. 2014. Front Immunol. 5:169.2. Zaki MH, et al. 2014. Proc. Natl. Acad. Sci. USA 111:385.3. Chen GY. 2014. Eur. J. Immunol. 44:321.4. Vladimer GI. 2013. Curr. Opin. Microbiol. 16:23.5. Allen IC, et al. 2012. Immunity 36:742.6. Vladimer GI, et al. 2012. Immunity 37:96.
Gene ID: 91662
UniProt: View information about NLRP12 on UniProt.org
Clone: 3D11A53
Regulatory Status: RUO
Other Names: NACHT, LRR and PYD domains-containing protein 12, Monarch-1, PYRIN-containing APAF1-like protein 7 (PYPAF7), Regulated by nitric oxide (RNO)
Isotype: Mouse IgG2b, κ