Biolegend, 677703, Direct-Blot™ HRP anti-mCherry Antibody, 100microl

mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins 1,2 . mCherry derives from proteins initially isolated from Cnidarians .
100microl
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: mCherry.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Product Citations: Irie F, et al. 2021. J Biol Chem. 296:100481. PubMed
RRID: AB_2566703 (BioLegend Cat. No. 677703)
Structure: 236 amino acids with a predicted molecular weight of approximately 27 kD.
Function: Fluorescent protein.
Biology Area: Cell Biology
Antigen References: 1. Shaner NC, et al. 2008. Nat. Methods 5:545. 2. Shaner NC, et al. 2005. Nat. Methods 2:905.
Gene ID: NA
UniProt: View information about mCherry on UniProt.org
Clone: 8C5.5
Regulatory Status: RUO
Other Names: DSRED
Isotype: Mouse IgG2a, κ