Biolegend, 682302, Purified anti-GALNT2 Antibody, 100μg

GALNT2 is a family member of polypeptide N-acetyl galactosaminyltransferases, which are responsible for initiating mucin type O-glycosylation by transferring GalNAc from UDP-GalNAc to Serine and Threonine residues of polypeptide acceptors. To date, there are about 20 members in this family of proteins. The characterization of peptide substrate specificity of each GALNT is critical in understanding their properties and biological roles. Trans-Golgi is the main subcellular location for GALNT2. This protein has a broad spectrum of peptide substrates such as EA2, Muc5AC, Muc1a, and Muc1b. GALNT2 may play a role in lipid metabolism. In a whole-body and liver-specific GALNT2 knockout mice study, it was shown that whole-body GALNT2 deficiency resulted in elevated VLDL-triglycerides (TG) levels as compared to wild type mice. GALNT2 KO mice did not exhibit differences in the postprandial TG clearance or VLDL-TG secretion. In mice with liver-specific deletion, reduced HDL-Cholesterol and reduced numbers of HDL particles with minimal changes in plasma TG or dietary TG clearance were observed. These findings indicate that GALNT2 may play a role as a regulator of plasma lipoprotein metabolism with tissue-specific regulatory roles.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human GALNT2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For immunocytochemistry, a concentration range of 0.1-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Goto A, et al. 2018. Traffic. 19:854. PubMed Pietrangelo A, et al. 2018. J Cell Sci. 131. PubMed
RRID: AB_2566611 (BioLegend Cat. No. 682302)
Structure: 519 amino acids with a predicted molecular weight of 60.3 kD.
Distribution: Golgi appartus and secreted.
Function: polypeptide N-acetyl-galactosaminyltransferases
Interaction: CCDC155 and NOD2.
Biology Area: Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: 1. White T, et al. 1995. J. Biol. Chem. 41:24156-65. 2. Schjoldager KT, Clausen H. 2012. Biochim. Biophys. Acta. 12:2079. 3. Gill DJ, et al. 2011. Trends Cell Biol. 3:149. 4. Gerken TA, et al. 2011. J. Biol. Chem. 286:14493. 5. Bennett EP, et al. 2012. Glycobiology 22:736. 6. Raman J, et al. 2012. Glycobiology 22:768.
Gene ID: 2590
UniProt: View information about GALNT2 on UniProt.org
Clone: 1501421
Regulatory Status: RUO
Other Names: Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc transferase 2, GalNAc-T2
Isotype: Mouse IgG2b, κ